Journal: Neurobiology of aging

Article Title: Life-long Overexpression of S100? in Down's Syndrome: Implications for Alzheimer Pathogenesis

doi:

Figure Lengend Snippet: Photomicrographs showing β-APP+ neurons in neocortex of young (Y) and adult (A) Down’s (DS) and control (C) patients (top four panels). Lower two panels show Tau2+ neurons in adult Down’s (A-DS) but not control (A-C) patients. Bars = 15 µm.

Article Snippet: Paraffin blocks were serially sectioned at a thickness of 10 µm, and immunoreaction of rabbit-anti-bovine S100β (a gift from Dr. Linda Van Eldik ( 21 )) and mouse-anti-human β-APP (clone 22C11, Boehringer-Mannheim) antibodies was performed on glass slide-mounted tissue sections as previously described ( 13 ).

Techniques:

Journal: Neurobiology of aging

Article Title: Life-long Overexpression of S100? in Down's Syndrome: Implications for Alzheimer Pathogenesis

doi:

Figure Lengend Snippet: Numerical density of β-APP+ neurons (A) and of Tau2+ neurons (B) in neocortex of Down’s and control patients. For β-APP+ neurons, the mean values were significantly different for young (* p < 0.02), and for adult (** p < 0.0001), but not for fetal age ranges. For Tau2+ neurons the mean values were significantly different for adults (** p < 0.0001).

Article Snippet: Paraffin blocks were serially sectioned at a thickness of 10 µm, and immunoreaction of rabbit-anti-bovine S100β (a gift from Dr. Linda Van Eldik ( 21 )) and mouse-anti-human β-APP (clone 22C11, Boehringer-Mannheim) antibodies was performed on glass slide-mounted tissue sections as previously described ( 13 ).

Techniques:

Journal: Neurobiology of aging

Article Title: Life-long Overexpression of S100? in Down's Syndrome: Implications for Alzheimer Pathogenesis

doi:

Figure Lengend Snippet: Dual plots of the numerical density of β-APP+ neurons vs. numerical density of S100β+ astrocytes for young and adult Down’s (●) and control (○) patients show a separation of the two patient populations. A regression line is shown for the Down’s syndrome values only. Values were significantly correlated for Down’s (r = 0.75, p < 0.05) but not for control patients.

Article Snippet: Paraffin blocks were serially sectioned at a thickness of 10 µm, and immunoreaction of rabbit-anti-bovine S100β (a gift from Dr. Linda Van Eldik ( 21 )) and mouse-anti-human β-APP (clone 22C11, Boehringer-Mannheim) antibodies was performed on glass slide-mounted tissue sections as previously described ( 13 ).

Techniques:

Journal: Acta Neuropathologica Communications

Article Title: Murine Aβ over-production produces diffuse and compact Alzheimer-type amyloid deposits

doi: 10.1186/s40478-015-0252-9

Figure Lengend Snippet: Comparison of APP and PS1 expression levels in PrP.MoAβ/PS1 and PrP.HuAβ/PS1 mice. The brains of 2 non-transgenic (age 1.6–2.4 months) and 3 each PrP.MoAβ/PS1 (Line D-943; aged 2.5–3.3 months) and PrP.HuAβ/PS1 (Line 85, aged 1.6–2.4 months) mice were homogenized in RIPA buffer, the supernatants were analyzed by immunoblotting 4–20% SDS-PAGE gels with antibodies to PS1 (1:5000, top row), human APP (mAb 6E10, 1:1500, second row), total APP (mAb 22C11, 1:1000, third row), or mouse SOD1 (1:2500, fourth row). The mouse SOD1 immunoblot served as a loading control. The levels of PS1dE9 and total APP were similar in the two lines of mice. Each lane contains 50 μg of total protein

Article Snippet: The following primary antibodies were used: rabbit anti-PS1NT antibody against PS1 (1:5000, kind gift of Dr. Gopal Thinakaran, University of Chicago, Chicago, IL, USA), 6E10 (mouse anti-human Aβ monoclonal, 1:1500, Covance, NJ), 22C11 (mouse anti-human and mouse APP N terminus monoclonal, 1:1000, EMD Millipore, MA) and human and mouse SOD1 antibody (m/hSOD1, rabbit polyclonal, 1:2500) [ ].

Techniques: Expressing, Transgenic Assay, Western Blot, SDS Page